Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![Alkali Scientific TG 10X Solution [TRIS Glycine Buffer 10X Solution], 1 Liter](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661625.jpg-250.jpg)
Alkali Scientific TG 10X Solution [TRIS Glycine Buffer 10X Solution], 1 Liter
TRIS Glycine Buffer 10X Solution is a high-performance buffer designed for use in electrophoresis, particularly for running SDS-PAGE gels. The 1-liter bottle of 10X solution allows for efficient dilution and preparation of multiple electrophoresis runs. This buffer system is well-suited for separating a wide range of proteins, providing reliable and consistent results. The TRIS-Glycine formulation is known for its excellent buffering capacity and is widely used in molecular biology and biochemistry for protein analysis. This buffer is compatible with numerous protein electrophoresis protocols, including those for high-precision applications such as Western blotting and protein quantification.
Nusep Inc NUVIEW UNIV GEL 10 10-WELL
NuSep's nUView Tris-Glycine SDS-PAGE Precast Gel 10% 10well. Cassette size: 10 x 8 cm. Fits Hoefer, Cytiva, Merck Tanks. 10 gel cassettes per box. All nUView™ precast gels incorporate a unique formulation allowing protein bands to be visualized in only 2 minutes in the presence of ultraviolet (UV) light. Simply place your gel over a 250-320 nm UV trans-illuminator and watch high-resolution bands appear in just 2 minutes. With nUView™ technology you no longer have to stain and de-stain your gels – increasing protein recovery and saving you significant time while receiving improved results.
Biotang Inc Phosphate Buffered RIPA with Glycerol (2X), 125 ml
Phosphate Buffered RIPA with Glycerol (2X), 125 ml.
Biotang Inc Dialysis tubing Storage buffer in PBS and Glycerol (autoclaved),; 125 ml
Dialysis tubing Storage buffer in PBS and Glycerol (autoclaved),; 125 ml.
Biotang Inc MOPS-EDTA-Sodium Acetate Buffer (MESA), 500 ml
MOPS-EDTA-Sodium Acetate Buffer (MESA), 500 ml.
![eMolecules 2306265-70-1 | tert-butyl 4-(hydroxymethyl)-2-azabicyclo[2.2.2]octane-2-carboxylate | 1g](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502159806.JPG-250.jpg)
eMolecules 2306265-70-1 | tert-butyl 4-(hydroxymethyl)-2-azabicyclo[2.2.2]octane-2-carboxylate | 1g
Medchem Express | Ginsenoside F1 | 5mg | 446275202 | HY-N0598 | 53963-43-2 | MFCD06410947 | 638.883 | C36H62O9
If you are unable to find the chemical you are looking for, make sure you are logged into your fishersci.com account and click on the following link:
eMolecules Building Block Tool
Teknova 2.5M Tris-HCl pH 7.5. Endotox
2.5M Tris-HCl, pH 7.5. Endotoxin Tested. 1000mL, Sterile. 6 pack.
Medchemexpress LLC Tris buffered saline | 10 TABLETS
Tris buffered saline is supplied as ready-to-dissolve tablets for preparing Tris/NaCl buffer solutions commonly used in molecular biology and protein applications. The tablet format simplifies buffer preparation and storage while delivering a stable pH environment for assays and electrophoresis.
- Ready-to-use tablet format for convenient buffer preparation.
- One tablet dissolves in 15 mL deionized water to yield 0.05 M Tris and 0.15 M sodium chloride.
- pH approximately 7.6 at 25 °C.
- White to off-white solid tablets for easy visual quality check.
- Suitable for DNA/RNA extraction, PCR, and protein electrophoresis.
- Compact packaging reduces storage space and eases transport.
Biotang Inc TE BUFFER, pH 8.0 autoclaved. Ready to use, 250 ml
TE BUFFER, pH 8.0 autoclaved. Ready to use, 250 ml.
LAB BIOREAGENTS LowBinding VI Buffer 500ml
Lab Bioreagents LowBindings (I, II, III, IV, V, VI, VII) are newly developed assay buffers that provides exceptional performance and broad compatibility for Western blot, ELISA, IHC and nucleic acid detection assays. The LowBindings helps to reduce the non-specific bindings, negative effects of interfering molecules, and cross-reactivities of antibodies and proteins from biomatrix. Therefore, LowBindings exhibit the low matrix interference and high signal-to-noise, by which the assay sensitivity and specificity have been significantly improved. As many factors are involved in immunoassays, there is no single assay buffer that is ideal for each assay. LowBindings (I, II, III, IV, V, VI, VII) are a variety of buffers based on different formulations.
Waters Corp Tris CaCl2 Buffer Salts, pH 7.5, 4/pk
Waters Corporation Tris CaCl2 Buffer Salts, pH 7.5, 4/pk
Crescent Chemical Co Inc TE BUFFER (100X) PH 8.0
TE Buffer (100X) pH 8.0 DNase/RNase not detected. 100 x concentrated aqueous solution. Size - 100ML Storage Conditions - +15 °C TO +30 °C Catalog Number - 39799.01
Bioworld TT - SDS Running Buffer (Anode) (10X), 500 mL
Product DescriptionTris-Tricine (TT)-SDS running buffer has a 10X concentration and a pH of 8.9. It contains 1M Tris-base, 1M Tricine and 1% SDS.The tricine system requires SDS in sample and running buffers for best results. Tricine-SDS PAGE is commonly used to separate proteins that range in molecular weight between 1 – 100 kDa but is preferred for the resolution of proteins smaller than 30 kDa.